Journal: Leukemia

Article Title: Guidelines for the characterization and publication of human malignant hematopoietic cell lines.

doi: 10.1038/sj.leu.2401428

Figure Lengend Snippet: Figure 1 Total numbers of published cell lines according to differ- ent cell line types from 1965 to 1998 (literature summary). The types of cell lines are explained in Table 2. In this graph are not included: Burkitt lymphoma cell lines, HTLV-I + ATL cell lines and EBV + B- LCLs. Shown are only the numbers for well-characterized cell lines. A considerable number of cell lines have been reported that are only incompletely described: B cell precursor, 27; B cell, 43; plasma cell, 38; immature T cell, 6; NK cell, 2; ALCL, 5; myelocytic, 18; mono- cytic, 4; erythroid–megakaryocytic, 1. A third cohort of cell lines is not characterized at all; the number of these uncharacterized cell lines is not known. ALCL, anaplastic large cell lymphoma; ATL, adult T cell leukemia; B-LCL, B lymphoblastoid cell line; EBV, Epstein–Barr virus; HTLV, human T cell leukemia virus; NK, natural killer.

Article Snippet: ALCL cell line SU-DHL-1 Dendritic lymphoid? mono-myeloid? dendritic cell lined —d Modified after Refs 1 and 4. aThe best known cell lines for each of these categories are often the ‘oldest’ cell lines (nearly all are available from cell line banks DSMZ or ATCC); see Ref. 1 for a historical perspective. bIt is often difficult to assign cell lines to either the erythrocytic or megakaryocytic cell lineage as most of these cell lines express features of both lineages, eg (hemo)globin, specific transcription factors, surface antigens, differentiation potential, etc; thus, it is preferable to use the term ‘erythrocytic–megakaryocytic cell line’. cSee Refs 13 and 14 for reviews on this controversial cell type. dAt present, no continuous human dendritic cell line has been published; see Refs 15 and 16 for reviews on this controversial cell type. genes.11 It appears to be 10- to 100-fold easier to establish an EBV + B-LCL from a patient with leukemia than a neoplastic leukemia cell line.12 The clear distinction between a MH cell line and an EBV + B-LCL is particularly relevant for cell lines established from patients with mature B cell malignancies such as chronic lymphocytic leukemias, hairy cell leukemia, B cell non-Hodgkin’s lymphomas, myeloma and other malignancies.

Techniques: Clinical Proteomics, Virus

Journal: Nature medicine

Article Title: Wiskott–Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma

doi: 10.1038/s41591-018-0262-9

Figure Lengend Snippet: (a) Western Blot analysis of ALK+ ALCL cell lines (SU-DHL1, JB6, Karpas-299 and DEL) transduced with doxycycline-inducible lentivirus co-expressing WASP and WIP (W&W) or a control reporter GFP (Ctrl). Black arrows: endogenous WASP and WIP; red arrows: Flag-tagged WASP and WIP. MAC-1 cell line and normal T cells were used as controls. The blot is representative of at least two independent experiments with similar results. Actin was used as a loading control. Uncropped blots are available in Supplementary Figure 11.

Article Snippet: Human ALK+ ALCL cell lines (TS, SU-DHL1, JB6, Karpas-299, DEL, SUP-M2 and L82) and ALK- cell lines (MAC-1, FePD and Jurkat) ALCL cell lines were obtained from DSMZ (German collection of Microorganisms and Cell Cultures).

Techniques: Western Blot, Transduction, Expressing, Control

Journal: Nature medicine

Article Title: Wiskott–Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma

doi: 10.1038/s41591-018-0262-9

Figure Lengend Snippet: (a) Western Blot performed on human ALK+ ALCL cells lines and ALK- T lymphoma lines or normal T cells blotted with the indicated antibodies. The blot is representative of two independent experiments with similar results. Actin was used as a loading control. Uncropped blots are available in Supplementary Figure 11.

Article Snippet: Human ALK+ ALCL cell lines (TS, SU-DHL1, JB6, Karpas-299, DEL, SUP-M2 and L82) and ALK- cell lines (MAC-1, FePD and Jurkat) ALCL cell lines were obtained from DSMZ (German collection of Microorganisms and Cell Cultures).

Techniques: Western Blot, Control

Journal: Nature medicine

Article Title: Wiskott–Aldrich syndrome protein (WASP) is a tumor suppressor in T cell lymphoma

doi: 10.1038/s41591-018-0262-9

Figure Lengend Snippet: (a) Gene-expression profiling analysis of ALK, WASP, WIP, N-WASP, CDC42 and TNFRS8 (CD30) on different cases of human T cell lymphomas: AITL, n = 40; PTCL-NOS, n = 74; ALK- ALCL, n = 24, ALK+ ALCL, n = 30. The boxes represent the first and third quartiles, and the line represents the median. The whiskers represent the upper and lower limits of the range (ALK+ ALCL vs AITL, ****P = 5.85 X 10−6; ALK+ ALCL vs PTCL-NOS, ****P = 6.08 X 10−12; ALK+ ALCL vs ALK- ALCL, **P = 0.0075; significance was determined by unpaired, two-tailed Student’s t-test). TNFRS8 (CD30) is strongly expressed in ALK- or ALK+ ALCL but not in other T cell lymphoma (TCL) subtypes.

Article Snippet: Human ALK+ ALCL cell lines (TS, SU-DHL1, JB6, Karpas-299, DEL, SUP-M2 and L82) and ALK- cell lines (MAC-1, FePD and Jurkat) ALCL cell lines were obtained from DSMZ (German collection of Microorganisms and Cell Cultures).

Techniques: Gene Expression, Two Tailed Test